Constructions of expression vectors of polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and transient expression of transgenes in immature oil palm embryos.
Identifieur interne : 000572 ( Main/Exploration ); précédent : 000571; suivant : 000573Constructions of expression vectors of polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and transient expression of transgenes in immature oil palm embryos.
Auteurs : Norazrin Ariffin [Malaisie] ; Ruslan Abdullah ; Mohd Rashdan Muad ; Juanita Lourdes ; Nurul Ain Emran ; Mohd Razi Ismail ; Ismanizan Ismail ; Mohd Fazli Mohd Fadzil ; Kong Lih Ling ; Yasmeen Siddiqui ; Anna Aryani Amir ; Zulkarami Berahim ; Mohd Husni OmarSource :
- Plasmid [ 1095-9890 ] ; 2011.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (métabolisme), Arecaceae (embryologie), Arecaceae (génétique), Arecaceae (métabolisme), Clonage moléculaire (MeSH), Graines (génétique), Graines (métabolisme), Ordre des gènes (MeSH), Plasmides (génétique), Polyesters (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Transformation génétique (MeSH), Transgènes (MeSH), Vecteurs génétiques (génétique).
- MESH :
- embryologie : Arecaceae.
- génétique : Agrobacterium tumefaciens, Arecaceae, Graines, Plasmides, Vecteurs génétiques.
- métabolisme : Agrobacterium tumefaciens, Arecaceae, Graines, Polyesters.
- Clonage moléculaire, Ordre des gènes, Régulation de l'expression des gènes végétaux, Transformation génétique, Transgènes.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (metabolism), Arecaceae (embryology), Arecaceae (genetics), Arecaceae (metabolism), Cloning, Molecular (MeSH), Gene Expression Regulation, Plant (MeSH), Gene Order (MeSH), Genetic Vectors (genetics), Plasmids (genetics), Polyesters (metabolism), Seeds (genetics), Seeds (metabolism), Transformation, Genetic (MeSH), Transgenes (MeSH).
- MESH :
- chemical , metabolism : Polyesters.
- embryology : Arecaceae.
- genetics : Agrobacterium tumefaciens, Arecaceae, Genetic Vectors, Plasmids, Seeds.
- metabolism : Agrobacterium tumefaciens, Arecaceae, Seeds.
- Cloning, Molecular, Gene Expression Regulation, Plant, Gene Order, Transformation, Genetic, Transgenes.
Abstract
Polyhydroxybutyrate-co-hydroxyvalerate (PHBV) is a polyhydroxyalkanoate (PHA) bioplastic group with thermoplastic properties is thus high in quality and can be degradable. PHBV can be produced by bacteria, but the process is not economically competitive with polymers produced from petrochemicals. To overcome this problem, research on transgenic plants has been carried out as one of the solutions to produce PHBV in economically sound alternative manner. Four different genes encoded with the enzymes necessary to catalyze PHBV are bktB, phaB, phaC and tdcB. All the genes came with modified CaMV 35S promoters (except for the tdcB gene, which was promoted by the native CaMV 35S promoter), nos terminator sequences and plastid sequences in order to target the genes into the plastids. Subcloning resulted in the generation of two different orientations of the tdcB, pLMIN (left) and pRMIN (right), both 17.557 and 19.967 kb in sizes. Both plasmids were transformed in immature embryos (IE) of oil palm via Agrobacterium tumefaciens. Assays of GUS were performed on one-week-old calli and 90% of the calli turned completely blue. This preliminary test showed positive results of integration. Six-months-old calli were harvested and RNA of the calli were isolated. RT-PCR was used to confirm the transient expression of PHBV transgenes in the calli. The bands were 258, 260, 315 and 200 bp in size for bktB, phaB, phaC and tdcB transgenes respectively. The data obtained showed that the bktB, phaB, phaC and tdcB genes were successfully integrated and expressed in the oil palm genome.
DOI: 10.1016/j.plasmid.2011.07.002
PubMed: 21827784
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(embryologie)</term><term>Arecaceae (génétique)</term><term>Arecaceae (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Graines (génétique)</term><term>Graines (métabolisme)</term><term>Ordre des gènes (MeSH)</term><term>Plasmides (génétique)</term><term>Polyesters (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Transgènes (MeSH)</term><term>Vecteurs génétiques (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Polyesters</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Arecaceae</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Arecaceae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arecaceae</term><term>Genetic Vectors</term><term>Plasmids</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Arecaceae</term><term>Graines</term><term>Plasmides</term><term>Vecteurs génétiques</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arecaceae</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Arecaceae</term><term>Graines</term><term>Polyesters</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Plant</term><term>Gene Order</term><term>Transformation, Genetic</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Ordre des gènes</term><term>Régulation de l'expression des gènes végétaux</term><term>Transformation génétique</term><term>Transgènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Polyhydroxybutyrate-co-hydroxyvalerate (PHBV) is a polyhydroxyalkanoate (PHA) bioplastic group with thermoplastic properties is thus high in quality and can be degradable. PHBV can be produced by bacteria, but the process is not economically competitive with polymers produced from petrochemicals. To overcome this problem, research on transgenic plants has been carried out as one of the solutions to produce PHBV in economically sound alternative manner. Four different genes encoded with the enzymes necessary to catalyze PHBV are bktB, phaB, phaC and tdcB. All the genes came with modified CaMV 35S promoters (except for the tdcB gene, which was promoted by the native CaMV 35S promoter), nos terminator sequences and plastid sequences in order to target the genes into the plastids. Subcloning resulted in the generation of two different orientations of the tdcB, pLMIN (left) and pRMIN (right), both 17.557 and 19.967 kb in sizes. Both plasmids were transformed in immature embryos (IE) of oil palm via Agrobacterium tumefaciens. Assays of GUS were performed on one-week-old calli and 90% of the calli turned completely blue. This preliminary test showed positive results of integration. Six-months-old calli were harvested and RNA of the calli were isolated. RT-PCR was used to confirm the transient expression of PHBV transgenes in the calli. The bands were 258, 260, 315 and 200 bp in size for bktB, phaB, phaC and tdcB transgenes respectively. The data obtained showed that the bktB, phaB, phaC and tdcB genes were successfully integrated and expressed in the oil palm genome.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21827784</PMID><DateCompleted><Year>2012</Year><Month>03</Month><Day>25</Day></DateCompleted><DateRevised><Year>2011</Year><Month>11</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-9890</ISSN><JournalIssue CitedMedium="Internet"><Volume>66</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Plasmid</Title><ISOAbbreviation>Plasmid</ISOAbbreviation></Journal><ArticleTitle>Constructions of expression vectors of polyhydroxybutyrate-co-hydroxyvalerate (PHBV) and transient expression of transgenes in immature oil palm embryos.</ArticleTitle><Pagination><MedlinePgn>136-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plasmid.2011.07.002</ELocationID><Abstract><AbstractText>Polyhydroxybutyrate-co-hydroxyvalerate (PHBV) is a polyhydroxyalkanoate (PHA) bioplastic group with thermoplastic properties is thus high in quality and can be degradable. PHBV can be produced by bacteria, but the process is not economically competitive with polymers produced from petrochemicals. To overcome this problem, research on transgenic plants has been carried out as one of the solutions to produce PHBV in economically sound alternative manner. Four different genes encoded with the enzymes necessary to catalyze PHBV are bktB, phaB, phaC and tdcB. All the genes came with modified CaMV 35S promoters (except for the tdcB gene, which was promoted by the native CaMV 35S promoter), nos terminator sequences and plastid sequences in order to target the genes into the plastids. Subcloning resulted in the generation of two different orientations of the tdcB, pLMIN (left) and pRMIN (right), both 17.557 and 19.967 kb in sizes. Both plasmids were transformed in immature embryos (IE) of oil palm via Agrobacterium tumefaciens. Assays of GUS were performed on one-week-old calli and 90% of the calli turned completely blue. This preliminary test showed positive results of integration. Six-months-old calli were harvested and RNA of the calli were isolated. RT-PCR was used to confirm the transient expression of PHBV transgenes in the calli. The bands were 258, 260, 315 and 200 bp in size for bktB, phaB, phaC and tdcB transgenes respectively. The data obtained showed that the bktB, phaB, phaC and tdcB genes were successfully integrated and expressed in the oil palm genome.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ariffin</LastName><ForeName>Norazrin</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Laboratory of Food Crops and Floriculture, Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. norazrin@putra.upm.edu.my</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abdullah</LastName><ForeName>Ruslan</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Rashdan Muad</LastName><ForeName>Mohd</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Lourdes</LastName><ForeName>Juanita</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Emran</LastName><ForeName>Nurul Ain</ForeName><Initials>NA</Initials></Author><Author ValidYN="Y"><LastName>Ismail</LastName><ForeName>Mohd Razi</ForeName><Initials>MR</Initials></Author><Author ValidYN="Y"><LastName>Ismail</LastName><ForeName>Ismanizan</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Fadzil</LastName><ForeName>Mohd Fazli Mohd</ForeName><Initials>MF</Initials></Author><Author ValidYN="Y"><LastName>Ling</LastName><ForeName>Kong Lih</ForeName><Initials>KL</Initials></Author><Author ValidYN="Y"><LastName>Siddiqui</LastName><ForeName>Yasmeen</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Amir</LastName><ForeName>Anna Aryani</ForeName><Initials>AA</Initials></Author><Author ValidYN="Y"><LastName>Berahim</LastName><ForeName>Zulkarami</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Husni Omar</LastName><ForeName>Mohd</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>08</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plasmid</MedlineTA><NlmUniqueID>7802221</NlmUniqueID><ISSNLinking>0147-619X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011091">Polyesters</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C052620">poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D028023" MajorTopicYN="N">Arecaceae</DescriptorName><QualifierName UI="Q000196" MajorTopicYN="N">embryology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D023061" MajorTopicYN="N">Gene Order</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011091" MajorTopicYN="N">Polyesters</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="Y">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>04</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2011</Year><Month>07</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>07</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21827784</ArticleId><ArticleId IdType="pii">S0147-619X(11)00058-8</ArticleId><ArticleId IdType="doi">10.1016/j.plasmid.2011.07.002</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Malaisie</li></country></list><tree><noCountry><name sortKey="Abdullah, Ruslan" sort="Abdullah, Ruslan" uniqKey="Abdullah R" first="Ruslan" last="Abdullah">Ruslan Abdullah</name><name sortKey="Amir, Anna Aryani" sort="Amir, Anna Aryani" uniqKey="Amir A" first="Anna Aryani" last="Amir">Anna Aryani Amir</name><name sortKey="Berahim, Zulkarami" sort="Berahim, Zulkarami" uniqKey="Berahim Z" first="Zulkarami" last="Berahim">Zulkarami Berahim</name><name sortKey="Emran, Nurul Ain" sort="Emran, Nurul Ain" uniqKey="Emran N" first="Nurul Ain" last="Emran">Nurul Ain Emran</name><name sortKey="Fadzil, Mohd Fazli Mohd" sort="Fadzil, Mohd Fazli Mohd" uniqKey="Fadzil M" first="Mohd Fazli Mohd" last="Fadzil">Mohd Fazli Mohd Fadzil</name><name sortKey="Husni Omar, Mohd" sort="Husni Omar, Mohd" uniqKey="Husni Omar M" first="Mohd" last="Husni Omar">Mohd Husni Omar</name><name sortKey="Ismail, Ismanizan" sort="Ismail, Ismanizan" uniqKey="Ismail I" first="Ismanizan" last="Ismail">Ismanizan Ismail</name><name sortKey="Ismail, Mohd Razi" sort="Ismail, Mohd Razi" uniqKey="Ismail M" first="Mohd Razi" last="Ismail">Mohd Razi Ismail</name><name sortKey="Ling, Kong Lih" sort="Ling, Kong Lih" uniqKey="Ling K" first="Kong Lih" last="Ling">Kong Lih Ling</name><name sortKey="Lourdes, Juanita" sort="Lourdes, Juanita" uniqKey="Lourdes J" first="Juanita" last="Lourdes">Juanita Lourdes</name><name sortKey="Rashdan Muad, Mohd" sort="Rashdan Muad, Mohd" uniqKey="Rashdan Muad M" first="Mohd" last="Rashdan Muad">Mohd Rashdan Muad</name><name sortKey="Siddiqui, Yasmeen" sort="Siddiqui, Yasmeen" uniqKey="Siddiqui Y" first="Yasmeen" last="Siddiqui">Yasmeen Siddiqui</name></noCountry><country name="Malaisie"><noRegion><name sortKey="Ariffin, Norazrin" sort="Ariffin, Norazrin" uniqKey="Ariffin N" first="Norazrin" last="Ariffin">Norazrin Ariffin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21827784" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |